Expand Long Template Pcr System

Expand long template pcr system is optimized to efficiently amplify large genomic dna fragments around 20 kb long.

Expand long template pcr system. This powerful polymerase mixture produces a high yield of pcr product from genomic dna. Expand long template pcr system is optimized to efficiently amplify large genomi c dna fragments around 20 kb long. Expand long template pcr system is a special enzyme mix that con tains thermostable taq dna polymerase and tgo dna polymerase 1 a thermostable dna polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of pcr product from genomic dna.

This powerful polymerase mixture produces a high yield of pcr product from genomic dna. This powerful polymerase mixture produces a high yield of pcr product from genomic dna. After this step do not place the buffers on ice. In addition buffers should be vortexed thoroughly.

Expand long template pcr system is optimized to efficiently amplify large genomic dna fragments around 20 kb long. Expand long template simplies long and accurate pcr reactions. The expand long range dntpack is the second generation version of the expand long template pcr system optimized and designed to consistently amplify pcr products from 5kb up to 25kb from genomic dna or up to 40kb from l dna templates. Expand long template pcr system is a unique enzyme mix that con tains thermostable taq dna polymerase and tgo dna polymerase 1 a thermostable dna polymerase with proofreading activity.

Using the expand long template pcr system always thaw and equilibrate all buffers at 37 to 56 0c before use. Enzymes in the expand long template pcr system were originally isolated from the thermophilic eubacteria thermus aquaticus taq bm and thermococcus gorgonarius tgo both expressed in e. Expand long template pcr system is a unique enzyme mix that con tains thermostable taq dna polymerase and tgo dna polymerase 1 a thermostable dna polymerase with proofreading activity. This opti mized enzyme blend and three buffer set produces pcr products from 5 to 20 kb in length.

80100 based on 1 pubmed citations. Roche expand long template polymerase chain reaction pcr system expand long template polymerase chain reaction pcr system supplied by roche used in various techniques. Im trying to amplify a 8kb fragment genepromoter form genomic dna using roche expand long template pcr system. My primers are 21bp long and the annealing temperature is 2 degree.

Even if taq were able to amplify such long fragments expand long tem plate would deliver twice the delity and much higher yields. The supplied buffer can be used for all pcr product sizes. Not detectable in up to 3 ml after 16 hours incubation at 650c.